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CD96 Antibody (630612) [Allophycocyanin]

Novus Biologicals, part of Bio-Techne | Catalog # FAB5690A

Novus Biologicals, part of Bio-Techne
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FAB5690A-0.1ml

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 630612

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD96
Val22-Met536
Accession # Q3U0X8

Specificity

Detects mouse CD96 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD96v2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD96 Antibody (630612) [Allophycocyanin]

Detection of CD96 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD96 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD96 APC-conjugated Monoclonal Antibody (Catalog # FAB5690A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for CD96 Antibody (630612) [Allophycocyanin]

Application
Recommended Usage

Flow Cytometry

10 uL/10^6 cells
Sample: See below

Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Preservative

Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily.  It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma delta T cells.  Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion.  Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein.  It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region.  The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355).  Unlike human, there is no splice variant in the second V-type domain.  There is, however, a potential isoform that shows a single Cys substitution for aa 437-602.  Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Alternate Names

CD96, TACTILE

Gene Symbol

CD96

UniProt

Additional CD96 Products

Product Documents for CD96 Antibody (630612) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD96 Antibody (630612) [Allophycocyanin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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