CD97 Antibody (MEM-180) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44881

Clone MEM-180 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-180

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD97 Antibodies »

Product Specifications

Immunogen

PHA-activated peripheral blood cells

Localization

Plasma membrane

Specificity

The antibody MEM-180 recognizes an unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes. HLDA VI; WS Code BP 415 HLDA VI; WS Code NL N-L023

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Scientific Data Images for CD97 Antibody (MEM-180) [FITC]

Flow Cytometry: CD97 Antibody (MEM-180) [FITC] [NBP1-44881] - Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.

Applications for CD97 Antibody (MEM-180) [FITC]

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD97

CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.

Alternate Names

CD97

Gene Symbol

ADGRE5

Additional CD97 Products

Product Documents for CD97 Antibody (MEM-180) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD97 Antibody (MEM-180) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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