CD97 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24552
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate
Cited:
Human
Predicted:
Chimpanzee (100%), Opossum (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
A portion of amino acids 220-270 of human Leukocyte Antigen CD97 was used as the immunogen
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD97 Antibody - BSA Free
Western Blot: CD97 Antibody [NBP2-24552]
Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.Immunohistochemistry-Paraffin: CD97 Antibody [NBP2-24552]
Immunohistochemistry-Paraffin: CD97 Antibody [NBP2-24552] - IHC analysis of human spleen using NBP2-24552 at 10 ug/ml.Simple Western: CD97 Antibody [NBP2-24552]
Simple Western: CD97 Antibody [NBP2-24552] - Simple Western lane view shows a specific band for CD97 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for CD97 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/ml
Simple Western
1:10
Western Blot
3-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 81 kDa.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 81 kDa.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.2 ml PBS and 0.2% Gelatin
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD97
Alternate Names
CD97
Gene Symbol
ADGRE5
Additional CD97 Products
Product Documents for CD97 Antibody - BSA Free
Product Specific Notices for CD97 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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