CD98 Antibody (HBJ127) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81108
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD98 Antibody (HBJ127) - Azide and BSA Free [NBP2-81108]](https://resources.bio-techne.com/images/products/CD98-Antibody-HBJ127-Immunocytochemistry-Immunofluorescence-NBP2-81108-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD98 Antibody (HBJ127) - Azide and BSA Free [NBP2-81108]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, ELISA, Functional, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # HBJ127
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
HBJ127 was obtained from hybridomas generated by the fusion of mouse myeloma cells and spleen cells from mice which had been immunized with T24 hyman bladder cancer cells.
Specificity
HBJ127 binds to the CD98 heavy chain at an epitope consisting of residues AFS (aa 442-444) (Itoh et al, 2007). The antibody has not been shown to cross-react with other species. CD98 is expressed at high levels on monocytes and at very low level on peripheral blood T and B lymphocytes, splenocytes, NK cells and granulocytes. CD98 is a disulfide-linked and glycosylated type II integral membrane protein. This protein has roles in normal and neoplastic cell growth and is required for the function of light chain amino acid transporters. It is also involved in sodium-independent transport of large neitral amino acids and in guiding and targeting LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, CD98 acts as an arginine/glutamine exchanger.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CD98 Antibody (HBJ127) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD98 Antibody (HBJ127) - Azide and BSA Free [NBP2-81108]
Immunocytochemistry/Immunofluorescence: CD98 Antibody (HBJ127) [NBP2-81108] - Immunofluorescence staining of fixed HeLa cells with [NBP2-81108]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of HBJ127 [NBP2-81108] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81108, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.Applications for CD98 Antibody (HBJ127) - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
HBJ127 can inhibit lymphocyte activation and proliferation in vitro (Yagita and Hashimoto, 1986), as well as inhibit the growth of human bladder tumors (Yagita, 1986). The antibody also mediates the inhibition of DNA synthesis of Con A-stimulated lymphocytes at concentrations greater than 13 ug/ml, the inhibition of mAB 6-1-13-induced c-src expression and the inhibition of mAb 6-1-13-induced polykaryocyte formation. In addition, HBJ127 can enhance expression of the mannose receptor on the surface of monocytes derived from healthy humans. HBJ127 shows positive staining of murine L929 cells transiently expressing human CD98 heavy chain in IF studies. The antibody can also be used to detect CD98 heavy chain by ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD98
Alternate Names
4F2, 4F2hc, CD98, CH98hc, FRP-1, SLC3A2
Gene Symbol
SLC3A2
Additional CD98 Products
Product Documents for CD98 Antibody (HBJ127) - Azide and BSA Free
Product Specific Notices for CD98 Antibody (HBJ127) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...