CD99 Antibody (12E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44694

Clone 12E7 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-44694-0.5ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12E7

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CD99 Antibodies »

Product Summary for CD99 Antibody (12E7)

Immunogen

Human acute lymphocytic leukemia T-cells

Localization

Cell surface

Marker

Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Tissue culture supernatant with 0.05% Azide.

Store at 2 to 8C.

Scientific Data Images for CD99 Antibody (12E7)

Immunohistochemistry-Paraffin: CD99 Antibody (12E7) [NBP2-44694]

Immunohistochemistry-Paraffin: CD99 Antibody (12E7) [NBP2-44694]

Immunohistochemistry-Paraffin: CD99 Antibody (12E7) [NBP2-44694] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).

Applications for CD99 Antibody (12E7)

Application
Recommended Usage

Flow Cytometry

5-10ul/million cells

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50-1:100
Application Notes
Immunohistochemistry (Formalin-fixed): 1:50-1:100 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

UniProt

P14209

Additional CD99 Products

Product Documents for CD99 Antibody (12E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD99 Antibody (12E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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