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CD99 Antibody (HO36-1.1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44692

Novus Biologicals, part of Bio-Techne
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NBP2-44692-0.1mg

Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # HO36-1.1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Purified E-rosette forming cells from human peripheral blood lymphocytes

Localization

Cell surface

Specificity

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Marker

Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for CD99 Antibody (HO36-1.1)

Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) [NBP2-44692]

Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) [NBP2-44692]

Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) [NBP2-44692] - Human Testicular Carcinoma stained with CD99 Monoclonal Antibody (HO36-1.1).
Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) [NBP2-44692]

Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) [NBP2-44692]

Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) [NBP2-44692] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (HO36-1.1).

Applications for CD99 Antibody (HO36-1.1)

Application
Recommended Usage

Flow Cytometry

5 - 10 ul/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:100
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein L purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (HO36-1.1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD99 Antibody (HO36-1.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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