CD99 Antibody (MEM-131)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-403

Clone MEM-131 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # MEM-131

Concentration

1 mg/ml

View all CD99 Antibodies »

Product Specifications

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Specificity

The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for CD99 Antibody (MEM-131)

Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403]

Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403]

Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the FITC conjugate of NB500-403. Staining of Human peripheral blood lymphocytes with HCD99 FITC.
Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403]

Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403]

Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the Biotin conjugate of NB500-403. Staining of Human peripheral blood lymphocytes biotinylated HCD99, followed by Sav-PE.

Applications for CD99 Antibody (MEM-131)

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blot: Use under non-reducing conditions. Antibody purity is > 95% (by SDS-PAGE).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

TBS (pH 8.0)

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD99

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (MEM-131)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD99 Antibody (MEM-131)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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