Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # MIC2/1495R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD99 Antibodies »

Product Summary for CD99 Antibody (MIC2/1495R) - Azide and BSA Free

Immunogen

Recombinant human CD99 protein (Uniprot: P14209)

Localization

Cell surface

Marker

Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53368).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD99 Antibody (MIC2/1495R) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/1495R) - Azide and BSA Free [NBP2-54373]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/1495R) - Azide and BSA Free [NBP2-54373]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/1495R) [NBP2-54373] - Formalin-fixed, paraffin-embedded human Ewing’s Sarcoma stained with CD99 Recombinant Rabbit Monoclonal Antibody (MIC2/1495R).

Applications for CD99 Antibody (MIC2/1495R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 - 10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 5-10ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (MIC2/1495R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD99 Antibody (MIC2/1495R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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