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CD99 Antibody (MIC2/3478R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07315

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # MIC2/3478R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Synthetic peptide corresponding to CD99 residues within aa85 C terminal of CD99 was used as an immunogen. (Uniprot: P14209 )

Localization

Cell Surface

Marker

Ewing s Sarcoma Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08589)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CD99 Antibody (MIC2/3478R)

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) [NBP3-07315]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) [NBP3-07315]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) [NBP3-07315] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/3478R).
Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) [NBP3-07315]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) [NBP3-07315]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) [NBP3-07315] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/3478R).

Applications for CD99 Antibody (MIC2/3478R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

UniProt

Additional CD99 Products

Product Documents for CD99 Antibody (MIC2/3478R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD99 Antibody (MIC2/3478R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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