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CD99 Antibody (MIC2/877)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44695

Novus Biologicals, part of Bio-Techne
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NBP2-44695-0.02mg
NBP2-44695-0.1mg

Key Product Details

Species Reactivity

Human, Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MIC2/877

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human CD99 protein (Uniprot: P14209)

Reactivity Notes

Does not react with Rat.

Localization

Cell surface

Marker

Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47895)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD99 Antibody (MIC2/877)

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Testicular Carcinoma & stained with CD99 Monoclonal Antibody (MIC2/877).
Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (MIC2/877).
Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695]

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/877) [NBP2-44695] - Human Ovarian Carcinoma & stained with CD99 Monoclonal Antibody (MIC2/877).

Applications for CD99 Antibody (MIC2/877)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

UniProt

Additional CD99 Products

Product Documents for CD99 Antibody (MIC2/877)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD99 Antibody (MIC2/877)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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