CD99 Antibody (OTI1D5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71197

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD99 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD99 Antibody (OTI1D5) - Azide and BSA Free

Western Blot: CD99 Antibody (OTI1D5)Azide and BSA Free [NBP2-71197]

Western Blot: CD99 Antibody (OTI1D5)Azide and BSA Free [NBP2-71197]

Western Blot: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99.
Immunohistochemistry: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197]

Immunohistochemistry: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197]

Immunohistochemistry: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197] - Analysis of Human pancreas tissue.
Flow Cytometry: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197]

Flow Cytometry: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197]

Flow Cytometry: CD99 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71197] - Analysis of Jurkat cells, using CD99 antibody (Red), compared to a nonspecific negative control antibody (Blue). Dilution: 1:50
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Applications for CD99 Antibody (OTI1D5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (OTI1D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD99 Antibody (OTI1D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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