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CD99-L2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85737

Novus Biologicals, part of Bio-Techne
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NBP1-85737
NBP1-85737-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FDDFNLEDAVKETSSVKQPWDHTTTTTTNRPGTTRAPAKPPGSGLDLADALDDQDDGRRKPGIGGRERWNHVTTTTKRPVTTRAPANTLGN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD99-L2 Antibody

Western Blot: CD99-L2 Antibody [NBP1-85737]

Western Blot: CD99-L2 Antibody [NBP1-85737]

Western Blot: CD99-L2 Antibody [NBP1-85737] - Analysis in control (vector only transfected HEK293T lysate) and CD99L2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CD99-L2 Antibody [NBP1-85737]

Immunocytochemistry/ Immunofluorescence: CD99-L2 Antibody [NBP1-85737]

Immunocytochemistry/Immunofluorescence: CD99-L2 Antibody [NBP1-85737] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD99-L2 Antibody [NBP1-85737]

Immunohistochemistry-Paraffin: CD99-L2 Antibody [NBP1-85737]

Immunohistochemistry-Paraffin: CD99-L2 Antibody [NBP1-85737] - Staining of human stomach, upper shows strong membranous and cytoplasmic positivity in glandular cells.

Applications for CD99-L2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD99-L2

CD99L2 may function as a homophilic adhesion molecule. Functions in leukocyte-endothelial cell interactions duringleukocyte extravasation, and in particular, at the diapedesis step. Does not seem to be involved in docking ofleukocytes to the vessel wall or in ly

Long Name

CD99 Molecule-like 2

Alternate Names

CD99B, CD99L2, MIC2L1

Gene Symbol

CD99L2

Additional CD99-L2 Products

Product Documents for CD99-L2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD99-L2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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