Cdc20 Antibody (rCDC20/7184)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13819

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rCDC20/7184

Concentration

0.2 mg/ml

View all Cdc20 Antibodies »

Product Summary for Cdc20 Antibody (rCDC20/7184)

Immunogen

Recombinant full-length human Cdc20 protein (Uniprot: Q12834 )

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14074)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cdc20 Antibody (rCDC20/7184)

Immunohistochemistry-Paraffin: Cdc20 Antibody (rCDC20/7184) [NBP3-13819]

Immunohistochemistry-Paraffin: Cdc20 Antibody (rCDC20/7184) [NBP3-13819]

Immunohistochemistry-Paraffin: Cdc20 Antibody (rCDC20/7184) [NBP3-13819] - Formalin-fixed, paraffin-embedded human kidney cancer stained with Cdc20 antibody (rCDC20/7184) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for Cdc20 Antibody (rCDC20/7184)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cdc20

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Long Name

Cell division cycle protein 20 homolog

Alternate Names

p55CDC

Gene Symbol

CDC20

UniProt

Q12834 (Human)

Additional Cdc20 Products

Product Documents for Cdc20 Antibody (rCDC20/7184)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cdc20 Antibody (rCDC20/7184)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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