CDC42 Antibody (3C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26670

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3C3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC42 Antibodies »

Product Summary for CDC42 Antibody (3C3)

Immunogen

A synthesized peptide derived from Human CDC42 [UniProt P60953]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42 Antibody (3C3)

CDC42 Antibody (3C3)

Immunohistochemistry: CDC42 Antibody (3C3) [NBP3-26670] -

Immunohistochemistry: CDC42 Antibody (3C3) [NBP3-26670] - Image of CDC42 Antibody (3C3) diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
CDC42 Antibody (3C3)

Western Blot: CDC42 Antibody (3C3) [NBP3-26670] -

Western Blot: CDC42 Antibody (3C3) [NBP3-26670] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse Brain whole cell lysate.
All lanes: CDC42 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 22, 22 kDa
Observed band size: 24 kDa

Applications for CDC42 Antibody (3C3)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CDC42

CDC42 is a small GTPase of the Rho subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc42, and is able to complement the yeast cdc42 1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott Aldrich syndrome protein (NWASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in at least two transcript variants.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Gene Symbol

CDC42

Additional CDC42 Products

Product Documents for CDC42 Antibody (3C3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDC42 Antibody (3C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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