CDC42EP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88381

Novus Biologicals, part of Bio-Techne
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NBP1-88381
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC42EP4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:PVKKANDGEGGDEEAGTEEAVPRRNGAAGPHSPDPLLDEQAFGDLTDLPVVPKATYGLKHAESIMSFHIDLGPSMLGDVLSIMDKEEWDPEEGEGGYHGDEGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42EP4 Antibody

Western Blot: CDC42EP4 Antibody [NBP1-88381]

Western Blot: CDC42EP4 Antibody [NBP1-88381]

Western Blot: CDC42EP4 Antibody [NBP1-88381] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CDC42EP4 Antibody [NBP1-88381]

Immunocytochemistry/ Immunofluorescence: CDC42EP4 Antibody [NBP1-88381]

Immunocytochemistry/Immunofluorescence: CDC42EP4 Antibody [NBP1-88381] - Staining of human cell line U-2 OS shows positivity in plasma membrane & actin filaments. Antibody staining is shown in green.

Applications for CDC42EP4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC42EP4

The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control. [provided by RefSeq]

Alternate Names

BORG4MGC3740, CDC42 effector protein (Rho GTPase binding) 4, cdc42 effector protein 4, CEP4Binder of Rho GTPases 4, KAIA1777, MGC17125

Gene Symbol

CDC42EP4

Additional CDC42EP4 Products

Product Documents for CDC42EP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDC42EP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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