CDC45L Antibody (3F11-1F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00008318-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F11-1F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC45L Antibodies »

Product Specifications

Immunogen

CDC45L (AAH06232, 1 a.a. ~ 566 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLEEEIVEQTMRRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS

Specificity

CDC45L - CDC45 cell division cycle 45-like (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDC45L Antibody (3F11-1F3)

Western Blot: CDC45L Antibody (3F11-1F3) [H00008318-M01]

Western Blot: CDC45L Antibody (3F11-1F3) [H00008318-M01]

Western Blot: CDC45L Antibody (3F11-1F3) [H00008318-M01] - Analysis of CDC45L expression in transfected 293T cell line by CDC45L monoclonal antibody (M01), clone 3F11-1F3.Lane 1: CDC45L transfected lysate(65.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CDC45L Antibody (3F11-1F3) [H00008318-M01]

Immunocytochemistry/ Immunofluorescence: CDC45L Antibody (3F11-1F3) [H00008318-M01]

Immunocytochemistry/Immunofluorescence: CDC45L Antibody (3F11-1F3) [H00008318-M01] - Analysis of monoclonal antibody to CDC45L on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: CDC45L Antibody (3F11-1F3) [H00008318-M01] - Detection limit for recombinant GST tagged CDC45L is approximately 3ng/ml as a capture antibody.

Applications for CDC45L Antibody (3F11-1F3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDC45L

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Alternate Names

CDC45 cell division cycle 45-like (S. cerevisiae), cell division cycle 45 homolog (S. cerevisiae), cell division cycle 45-like 2, human CDC45, S.cerevisiae, homolog)-like

Entrez Gene IDs

8318 (Human)

Gene Symbol

CDC45

OMIM

603465 (Human)

UniProt

AAH06232 (Human)

Additional CDC45L Products

Product Documents for CDC45L Antibody (3F11-1F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDC45L Antibody (3F11-1F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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