CDC45L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32772

Novus Biologicals, part of Bio-Techne
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NBP1-32772
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (90%), Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC45L Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC45L. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC45L Antibody

Western Blot: CDC45L Antibody [NBP1-32772]

Western Blot: CDC45L Antibody [NBP1-32772]

Western Blot: CDC45L Antibody [NBP1-32772] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CDC45L Antibody [NBP1-32772]

Immunocytochemistry/ Immunofluorescence: CDC45L Antibody [NBP1-32772]

Immunocytochemistry/Immunofluorescence: CDC45L Antibody [NBP1-32772] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC45L protein stained by CDC45L antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Applications for CDC45L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDC45L

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Alternate Names

CDC45 cell division cycle 45-like (S. cerevisiae), cell division cycle 45 homolog (S. cerevisiae), cell division cycle 45-like 2, human CDC45, S.cerevisiae, homolog)-like

Gene Symbol

CDC45

Additional CDC45L Products

Product Documents for CDC45L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC45L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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