Cdc6 [p Ser54] Antibody (JE59-52)

Western Blot: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174]

Western Blot: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 63 kDa
Observed band size: 63 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32174) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174]

Immunohistochemistry: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32174) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.