Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CDC73 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Parafibromin using the numbering given in TrEMBL entry Q6P1J9 (GeneID 79577).

Specificity

NB200-184 is specific for human Parafibromin protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC73/HRPT2 Antibody

Western Blot: CDC73/HRPT2 Antibody [NB200-184]

Western Blot: CDC73/HRPT2 Antibody [NB200-184]

Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184]

Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184]

Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CDC73/HRPT2 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:5000-1:20000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDC73

Parafibromin, the product of gene HPRT2, acts as a tumor suppressor protein by inhibiting cell proliferation and blocking expression of cyclin D1 when overexpressed. Parafibromin has also been shown to bind with the PAF1 complex / RNA polymerase II which is involved with transcription elongation and the RNA processing pathway.

Long Name

Cell Division Cycle 73

Alternate Names

HPT-JT, HRPT2, Parafibromin

Entrez Gene IDs

79577 (Human)

Gene Symbol

CDC73

UniProt

Q6P1J9

Additional CDC73 Products

Product Documents for CDC73/HRPT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDC73/HRPT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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