CDK2 [p Tyr15] Antibody (2C4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26428
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2C4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human CDK2 [p Tyr15] [UniProt P24941]
Modification
p Tyr 15
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CDK2 [p Tyr15] Antibody (2C4)
Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] -
Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).
Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).
Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] -
Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not).All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 34 KDa
Observed band size: 34 KDa
Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] -
Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.Applications for CDK2 [p Tyr15] Antibody (2C4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: CDK2
Long Name
Cyclin-dependent Kinase 2
Alternate Names
cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)
Gene Symbol
CDK2
Additional CDK2 Products
Product Documents for CDK2 [p Tyr15] Antibody (2C4)
Product Specific Notices for CDK2 [p Tyr15] Antibody (2C4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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