CDK5RAP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86780

Novus Biologicals, part of Bio-Techne
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NBP1-86780
NBP1-86780-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDK5RAP3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LIGKLTSLQLQHLFMILASPRYVDRVTEFLQQKLKQSQLLALKKELMVQKQQEALEEQAALEPKLDLLLEKTKELQKLIEADISKRYSGRPVNLMGTSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDK5RAP3 Antibody

Western Blot: CDK5RAP3 Antibody [NBP1-86780]

Western Blot: CDK5RAP3 Antibody [NBP1-86780]

Western Blot: CDK5RAP3 Antibody [NBP1-86780] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CDK5RAP3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CDK5RAP3 Antibody [NBP1-86780]

Immunocytochemistry/ Immunofluorescence: CDK5RAP3 Antibody [NBP1-86780]

Immunocytochemistry/Immunofluorescence: CDK5RAP3 Antibody [NBP1-86780] - Staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Immunohistochemistry-Paraffin: CDK5RAP3 Antibody [NBP1-86780]

Immunohistochemistry-Paraffin: CDK5RAP3 Antibody [NBP1-86780]

Immunohistochemistry-Paraffin: CDK5RAP3 Antibody [NBP1-86780] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
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Applications for CDK5RAP3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK5RAP3

Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein, which may be a substrate of neuronal CDC2-like kinase, has also been found in vascular endothelial cells, where it mediates cell proliferation.

Alternate Names

C53, CDK5 activator-binding protein C53, CDK5 regulatory subunit associated protein 3, FLJ13660, HSF-27, IC53CDK5 regulatory subunit-associated protein 3, ischemic heart CDK5 activator-binding protein C53, LXXLL/leucine-zipper-containing ARFbinding protein, LZAP, MST016, OK/SW-cl.114, Protein HSF-27

Gene Symbol

CDK5RAP3

Additional CDK5RAP3 Products

Product Documents for CDK5RAP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDK5RAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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