Cdk9 Antibody (2D7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001025-M07

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Drosophila

Cited:

Insect - Drosophila

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cdk9 Antibodies »

Product Specifications

Immunogen

CDK9 (AAH01968, 271 a.a. ~ 372 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 28754981).

Specificity

CDK9 - cyclin-dependent kinase 9 (CDC2-related kinase) (2D7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cdk9 Antibody (2D7)

Western Blot: Cdk9 Antibody (2D7) [H00001025-M07]

Western Blot: Cdk9 Antibody (2D7) [H00001025-M07]

Western Blot: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of CDK9 expression in transfected 293T cell line by CDK9 monoclonal antibody (M07), clone 2D7. Lane 1: CDK9 transfected lysatE (42.778 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (2D7) [H00001025-M07]

Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (2D7) [H00001025-M07]

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of monoclonal antibody to CDK9 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: Cdk9 Antibody (2D7) [H00001025-M07]

ELISA: Cdk9 Antibody (2D7) [H00001025-M07]

ELISA: Cdk9 Antibody (2D7) [H00001025-M07] - Detection limit for recombinant GST tagged CDK9 is approximately 1ng/ml as a capture antibody.

Applications for Cdk9 Antibody (2D7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.Use in immunoprecipitation reported in scientific literature (PMID 28754981).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cdk9

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq]

Long Name

Cyclin-dependent kinase 9

Alternate Names

C-2K, CDC2L4, TAK

Entrez Gene IDs

1025 (Human)

Gene Symbol

CDK9

UniProt

AAH01968 (Human)

Additional Cdk9 Products

Product Documents for Cdk9 Antibody (2D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cdk9 Antibody (2D7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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