Cdk9 [p Thr29] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77756

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cdk9 Antibodies »

Product Specifications

Immunogen

This affinity purified Cdk9 [p Thr29] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T29 in the human Cdk9 protein. (Uniprot: P50750)

Reactivity Notes

Cross-reactivity with CDK9 from other sources has not been determined

Modification

p Thr29

Specificity

This antibody is specific for human CDK9 protein phosphorylated at T29. A BLAST analysis was used to suggest cross-reactivity with CDK9 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with CDK9 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cdk9 [p Thr29] Antibody

Western Blot: Cdk9 [p Thr29] Antibody [NBP1-77756]

Western Blot: Cdk9 [p Thr29] Antibody [NBP1-77756]

Western Blot: Cdk9 [p Thr29] Antibody [NBP1-77756] - Shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution.
Cdk9 [p Thr29] Antibody

Cdk9 [p Thr29] Antibody

Western blot using affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.

Applications for Cdk9 [p Thr29] Antibody

Application
Recommended Usage

ELISA

1:5000-1:24000

Immunoprecipitation

1:100

Western Blot

1:200-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kDa in size corresponding to phosphorylated CDK9 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human CDK9 pT29 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Cdk9

CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family.? CDK family members are highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2 and are known as important cell cycle regulators.? CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. ?This kinase was also found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with, and is regulated by, its regulatory subunit, cyclin T or cyclin K. HIV-1 Tat protein.

Long Name

Cyclin-dependent kinase 9

Alternate Names

C-2K, CDC2L4, TAK

Gene Symbol

CDK9

UniProt

P50750 (Human), NC_000009.11 (Human)

Additional Cdk9 Products

Product Documents for Cdk9 [p Thr29] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cdk9 [p Thr29] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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