CDYL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34011

Novus Biologicals, part of Bio-Techne
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NBP2-34011
NBP2-34011-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDYL Antibodies »

Product Summary for CDYL Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PVKSRTAVDGFQSESPEKLDPVEQGQEDTVAPEVAAEKPVGALLGPGAERARMGSRPRIHP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDYL Antibody

Western Blot: CDYL Antibody [NBP2-34011]

Western Blot: CDYL Antibody [NBP2-34011]

Western Blot: CDYL Antibody [NBP2-34011] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: CDYL Antibody [NBP2-34011]

Immunocytochemistry/ Immunofluorescence: CDYL Antibody [NBP2-34011]

Immunocytochemistry/Immunofluorescence: CDYL Antibody [NBP2-34011] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011]

Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011]

Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
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Applications for CDYL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDYL

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.

Alternate Names

CDYL1bA620A17.2 (chromodomain protein, Y chromosome-like), CDY-like, CDY-like, autosomal, chromodomain protein, Y chromosome-like, chromodomain protein, Y-like, chromodomain Y-like protein, DKFZp586C1622, EC 2.3.1.48, MGC131936, testis-specific chromodomain Y-like protein

Gene Symbol

CDYL

UniProt

Q9Y232 (Human)

Additional CDYL Products

Product Documents for CDYL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDYL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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