CEA Antibody (PARLAM 4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97718
![Immunohistochemistry-Paraffin: CEA Antibody (PARLAM 4) - BSA Free [NBP1-97718]](https://resources.bio-techne.com/images/products/CEA-Antibody-PARLAM-4-Immunohistochemistry-Paraffin-NBP1-97718-img0001.jpg "Immunohistochemistry-Paraffin: CEA Antibody (PARLAM 4) - BSA Free [NBP1-97718]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PARLAM 4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody is a mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Ag 14 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with isolated Human CEA. The immunogen has been isolated from Human colonic carcinoma cells.
Specificity
Most polyclonal CEA antisera show cross-reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross-reacting antigen 1/11 (NCA). This antibody does not show cross reactivity, neither with BGP nor with NCA. In immunoblotting the antibody recognizes a single band of 180 kD.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Scientific Data Images for CEA Antibody (PARLAM 4) - BSA Free
Immunohistochemistry-Paraffin: CEA Antibody (PARLAM 4) - BSA Free [NBP1-97718]
Immunohistochemistry-Paraffin: CEA Antibody (PARLAM 4) [NBP1-97718] - Human head and neck squamous cell carcinoma section. Antigen retrieval at pH 9. CEA antibody at 1:100. IHC-P image submitted by a verified customer review.Applications for CEA Antibody (PARLAM 4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:25-1:100
Immunohistochemistry-Paraffin
1:25-1:100
Western Blot
1:100-1:500
Application Notes
This antibody is suitable for flow cytometry, immunoblotting, immunocytochemistry on methanol fixed cells and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. The antibody is also reactive in formalin-fixed and paraffin-embedded tissue sections after treatment with citrate buffer pH 6.0 in an autoclave. Human colon carcinoma tissue is used as positive control. Optimal antibody dilution should be determined by titration; we recommend a 1:25 - 1:100 dilution for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting application.
This antibody is CyTOF ready..
This antibody is CyTOF ready..
Reviewed Applications
Read 1 review rated 5 using NBP1-97718 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH 7.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CEA
Alternate Names
Carcino Embryonic Antigen, Carcinoembryonic antigen
Additional CEA Products
Product Documents for CEA Antibody (PARLAM 4) - BSA Free
Product Specific Notices for CEA Antibody (PARLAM 4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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