CEACAM1/CD66a Antibody (103)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89666

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89666-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 103

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CEACAM-1/CD66a Antibodies »

Product Summary for CEACAM1/CD66a Antibody (103)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM1/CD66a (Accession#: NP_001020083.1; Met1-Gly428).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CEACAM1/CD66a Antibody (103)

Immunocytochemistry/ Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666]

Immunocytochemistry/ Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666]

Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Staining of CEACAM1 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CEACAM1 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
Flow Cytometry: CEACAM1/CD66a Antibody (103) [NBP2-89666]

Flow Cytometry: CEACAM1/CD66a Antibody (103) [NBP2-89666]

Flow Cytometry: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Analysis of Human CEACAM1(CD66a) expression in HT29 cells. The cells were harvested and stained with purified Rabbit anti-human CEACAM1, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD (TM) FACSCalibur flow cytometry system.

Applications for CEACAM1/CD66a Antibody (103)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-1/CD66a

CEACAM1 encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined. [provided by RefSeq]

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1

Alternate Names

BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR

Gene Symbol

CEACAM1

Additional CEACAM-1/CD66a Products

Product Documents for CEACAM1/CD66a Antibody (103)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CEACAM1/CD66a Antibody (103)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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