CEACAM1/CD66a Antibody (BLR032F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76405

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76405
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR032F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CEACAM-1/CD66a Antibodies »

Product Summary for CEACAM1/CD66a Antibody (BLR032F)

Immunogen

Recombinant protein representing the extracellular domain of human CEACAM1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEACAM1/CD66a Antibody (BLR032F)

Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]

Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]

Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 by WB of UACC-257, SK-MEL-28, Hep-G2, SUP-M2, HeLa, HEK293T, Jurkat, RAW 264.7, and TCMK-1 lysate. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-Actin.
Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]

Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]

Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 in FFPE HT-29 cells by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]

Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]

Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 in FFPE colon carcinoma by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for CEACAM1/CD66a Antibody (BLR032F)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000
Application Notes
IHC-P-. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC/IF-Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CEACAM-1/CD66a

Carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM1 mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to CEACAM1 including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1

Alternate Names

BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR

Gene Symbol

CEACAM1

Additional CEACAM-1/CD66a Products

Product Documents for CEACAM1/CD66a Antibody (BLR032F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEACAM1/CD66a Antibody (BLR032F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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