CEACAM5/CD66e Antibody (5C8C4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54625
![Immunoprecipitation: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]](https://resources.bio-techne.com/images/products/CEACAM-5-CD66e-Antibody-5C8C4-Immunoprecipitation-NBP2-54625-img0006.jpg "Immunoprecipitation: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]")
![Western Blot: CEACAM5/CD66e Antibody (5C8C4)BSA Free [NBP2-54625]](https://resources.bio-techne.com/images/products/CEACAM-5-CD66e-Antibody-5C8C4-Western-Blot-NBP2-54625-img0008.jpg "Western Blot: CEACAM5/CD66e Antibody (5C8C4)BSA Free [NBP2-54625]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5C8C4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Soluble human CEACAM5/CD66e
Specificity
This antibody recognizes human CEACAM 5. It does not cross-react with CEACAM-1, -3, -4, -6, -7, and -8 or rat CEACAM1, mouse CEACAM1 and mouse CEACAM2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CEACAM5/CD66e Antibody (5C8C4) - BSA Free
Western Blot: CEACAM5/CD66e Antibody (5C8C4)BSA Free [NBP2-54625]
Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.![Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]](https://resources.bio-techne.com/images/products/CEACAM-5-CD66e-Antibody-5C8C4-Flow-Cytometry-NBP2-54625-img0003.jpg "Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]")
Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]
Flow Cytometry: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM5 [5C8C4] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).![ELISA: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]](https://resources.bio-techne.com/images/products/CEACAM-5-CD66e-Antibody-5C8C4-ELISA-NBP2-54625-img0002.jpg "ELISA: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]")
ELISA: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]
ELISA: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.Applications for CEACAM5/CD66e Antibody (5C8C4) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CEACAM-5/CD66e
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Alternate Names
CD66e, CEA, CEACAM5
Gene Symbol
CEACAM5
Additional CEACAM-5/CD66e Products
Product Documents for CEACAM5/CD66e Antibody (5C8C4) - BSA Free
Product Specific Notices for CEACAM5/CD66e Antibody (5C8C4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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