Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # By114

Format

BSA Free

Concentration

1 mg/ml

View all CEACAM-5/CD66e Antibodies »

Product Specifications

Immunogen

B cell Lymphoma cells

Specificity

This antibody recognizes human CEACAM5 and CEACAM6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for CEACAM5/CD66e Antibody (By114) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1 - 2 ug/ml

Western Blot

1:100 - 1:1000
Application Notes
Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. Recommended for solid phase ELISA. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-5/CD66e

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Gene Symbol

CEACAM5

Additional CEACAM-5/CD66e Products

Product Documents for CEACAM5/CD66e Antibody (By114) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CEACAM5/CD66e Antibody (By114) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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