CEACAM5/CD66e Antibody (CB30) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-58734
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CB30
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human carcinoembryonic antigen (CEA; CEACAM5)
Specificity
The antibody CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CEACAM5/CD66e Antibody (CB30) - BSA Free
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]
Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinomaImmunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]
Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinomaApplications for CEACAM5/CD66e Antibody (CB30) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
1:50
Application Notes
For paraffin sections use standard ABC technique with pretreatment in 0.1% pepsin in 0.1M HCl for 30 minutes at RT. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CEACAM-5/CD66e
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Alternate Names
CD66e, CEA, CEACAM5
Entrez Gene IDs
1048 (Human)
Gene Symbol
CEACAM5
Additional CEACAM-5/CD66e Products
Product Documents for CEACAM5/CD66e Antibody (CB30) - BSA Free
Product Specific Notices for CEACAM5/CD66e Antibody (CB30) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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