CEACAM6/CD66c Antibody (408)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89669
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 408
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM6/CD66c (Accession#: NP_002474.4; Met 1-Gly 320).
Specificity
No cross-reactivity in ELISA with: Human CEACAM1 / CD66aHuman CEACAM5 / CD66e
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CEACAM6/CD66c Antibody (408)
Immunocytochemistry/ Immunofluorescence: CEACAM6/CD66c Antibody (408) [NBP2-89669]
Immunocytochemistry/Immunofluorescence: CEACAM6/CD66c Antibody (408) [NBP2-89669] - Staining of Human CEACAM6 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CEACAM6 monoclonal antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Flow Cytometry: CEACAM6/CD66c Antibody (408) [NBP2-89669]
Flow Cytometry: CEACAM6/CD66c Antibody (408) [NBP2-89669] - Analysis of Human CEACAM6 expression on human peripheral blood granulocytes. Cells were stained with purified anti-Human CEACAM6, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.Applications for CEACAM6/CD66c Antibody (408)
Application
Recommended Usage
ELISA
1:25000-1:50000
Flow Cytometry
1:100-1:500
Application Notes
This antibody will detect Human CEACAM6/CD66c in ELISA pair set (Catalog: # NBP2-79288). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89672).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CEACAM-6/CD66c
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 6
Alternate Names
CD66c, CEACAM6, CEAL, NCA
Gene Symbol
CEACAM6
Additional CEACAM-6/CD66c Products
Product Documents for CEACAM6/CD66c Antibody (408)
Product Specific Notices for CEACAM6/CD66c Antibody (408)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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