CEACAM6/CD66c Antibody (408)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89669

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 408

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CEACAM-6/CD66c Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM6/CD66c (Accession#: NP_002474.4; Met 1-Gly 320).

Specificity

No cross-reactivity in ELISA with: Human CEACAM1 / CD66aHuman CEACAM5 / CD66e

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CEACAM6/CD66c Antibody (408)

Immunocytochemistry/ Immunofluorescence: CEACAM6/CD66c Antibody (408) [NBP2-89669]

Immunocytochemistry/ Immunofluorescence: CEACAM6/CD66c Antibody (408) [NBP2-89669]

Immunocytochemistry/Immunofluorescence: CEACAM6/CD66c Antibody (408) [NBP2-89669] - Staining of Human CEACAM6 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CEACAM6 monoclonal antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Flow Cytometry: CEACAM6/CD66c Antibody (408) [NBP2-89669]

Flow Cytometry: CEACAM6/CD66c Antibody (408) [NBP2-89669]

Flow Cytometry: CEACAM6/CD66c Antibody (408) [NBP2-89669] - Analysis of Human CEACAM6 expression on human peripheral blood granulocytes. Cells were stained with purified anti-Human CEACAM6, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.

Applications for CEACAM6/CD66c Antibody (408)

Application
Recommended Usage

ELISA

1:25000-1:50000

Flow Cytometry

1:100-1:500
Application Notes
This antibody will detect Human CEACAM6/CD66c in ELISA pair set (Catalog: # NBP2-79288). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89672).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-6/CD66c

CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 6

Alternate Names

CD66c, CEACAM6, CEAL, NCA

Gene Symbol

CEACAM6

Additional CEACAM-6/CD66c Products

Product Documents for CEACAM6/CD66c Antibody (408)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEACAM6/CD66c Antibody (408)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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