CEBP alpha Antibody (15C8)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1438

Novus Biologicals, part of Bio-Techne
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NB600-1438
NB600-1438-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 15C8

Concentration

1 mg/ml

View all CEBP alpha Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.

Epitope

Amino terminal region.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CEBP alpha Antibody (15C8)

Western Blot: CEBP alpha Antibody (15C8) [NB600-1438]

Western Blot: CEBP alpha Antibody (15C8) [NB600-1438]

Western Blot: CEBP alpha Antibody (15C8) [NB600-1438] - Analysis of transfected 293T cells.

Applications for CEBP alpha Antibody (15C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

2 ug/ml
Application Notes
ICC usage was reported in scientific literature (PMID: 20075868).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CEBP alpha

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Mutations in this gene are frequently found in acute myeloid leukemia (AML).

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Entrez Gene IDs

1050 (Human); 12606 (Mouse); 24252 (Rat)

Gene Symbol

CEBPA

UniProt

P49715 (Human)

Additional CEBP alpha Products

Product Documents for CEBP alpha Antibody (15C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEBP alpha Antibody (15C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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