CEBP alpha Antibody (HL1747) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25386
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (100%), Mouse (93%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1747
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for CEBP alpha Antibody (HL1747) - Azide and BSA Free
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CEBP alpha. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CEBP alpha Antibody (HL1747) - Azide and BSA Free
Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] -
Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] -
Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] -
Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - C/EBP alpha antibody [HL1747] detects C/EBP alpha protein at nucleus by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: C/EBP alpha stained by C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:500. Blue: Fluoroshield with DAPI .Applications for CEBP alpha Antibody (HL1747) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CEBP alpha
Long Name
CCAAT/enhancer-Binding Protein alpha
Alternate Names
C/EBP-alpha, CEBPA
Gene Symbol
CEBPA
Additional CEBP alpha Products
Product Documents for CEBP alpha Antibody (HL1747) - Azide and BSA Free
Product Specific Notices for CEBP alpha Antibody (HL1747) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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