Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CEBP Beta Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 50 and 100 of human CCAAT/Enhancer Binding Protein (C/EBP), beta using the numbering given in entry NP_005185.2 (GeneID 1051).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEBP Beta Antibody

Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179]

Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179]

Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .
Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody NBP1-46180, which recognizes a downstream epitope.

Applications for CEBP Beta Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CEBP Beta

NF-IL6 is a member of the CAAT/enhancer-binding protein (C/EBP) family of transcription factors. It has been shown to directly interact with the retinoblastoma (RB) protein.

Alternate Names

C/EBP beta, C/EBP-beta, CCAAT/enhancer binding protein (C/EBP), beta, CCAAT/enhancer-binding protein beta, CRP2, IL6DBP, interleukin 6-dependent DNA-binding protein, LAPTCF-5, Liver activator protein, liver-enriched transcriptional activator protein, MGC32080, NF-IL6, Nuclear factor NF-IL6, nuclear factor of interleukin 6, TCF5NFIL6, Transcription factor 5

Gene Symbol

CEBPB

Additional CEBP Beta Products

Product Documents for CEBP Beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CEBP Beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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