CEBP gamma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36944

Novus Biologicals, part of Bio-Techne
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NBP1-36944
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CEBP gamma Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ENTTADGDNAGQ corresponding to C-Terminus according to NP_001797.1.

Epitope

C Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CEBP gamma Antibody

Immunohistochemistry-Paraffin: CEBP gamma Antibody [NBP1-36944]

Immunohistochemistry-Paraffin: CEBP gamma Antibody [NBP1-36944]

Immunohistochemistry-Paraffin: CEBP gamma Antibody [NBP1-36944] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CEBP gamma Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:2000

Western Blot

1 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx. 26kDa band in Human Lung lysates and in lysates of cell lines A549 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 16.4kDa according to NP_001797.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cortex).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CEBP gamma

The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP episilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

Alternate Names

c/EBP gamma, CCAAT/enhancer binding protein (C/EBP), gamma, CCAAT/enhancer-binding protein gamma, GPE1BP, IG/EBP-1

Entrez Gene IDs

1054 (Human)

Gene Symbol

CEBPG

UniProt

NP_001797.1 (Human)

Additional CEBP gamma Products

Product Documents for CEBP gamma Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CEBP gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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