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CELA1 Antibody (4H5)

Novus Biologicals, part of Bio-Techne | Catalog # H00001990-M02

Novus Biologicals, part of Bio-Techne
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H00001990-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ELA1 (NP_001962.3, 159 a.a. ~ 257 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAQTLQQAYLPSVDYAICSSSSYWGSTVKNTMVCAGGDGVRSGCQGDSGGPLHCLVNGKYSVHGVTSFVSSRGCNVSRKPTVFTQVSAYISWINNVIAS

Specificity

Reacts with elastase 1, pancreatic.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CELA1 Antibody (4H5)

Immunocytochemistry/ Immunofluorescence: CELA1 Antibody (4H5) [H00001990-M02]

Immunocytochemistry/ Immunofluorescence: CELA1 Antibody (4H5) [H00001990-M02]

Immunocytochemistry/Immunofluorescence: CELA1 Antibody (4H5) [H00001990-M02] - Analysis of monoclonal antibody to ELA1 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CELA1 Antibody (4H5) [H00001990-M02]

Immunohistochemistry-Paraffin: CELA1 Antibody (4H5) [H00001990-M02]

Immunohistochemistry-Paraffin: CELA1 Antibody (4H5) [H00001990-M02] - Analysis of monoclonal antibody to ELA1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
Sandwich ELISA: CELA1 Antibody (4H5) [H00001990-M02] - Detection limit for recombinant GST tagged ELA1 is 3 ng/ml as a capture antibody.

Applications for CELA1 Antibody (4H5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Reviewed Applications

Read 1 review rated 3 using H00001990-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CELA1

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq]

Alternate Names

chymotrypsin-like elastase family member 1, chymotrypsin-like elastase family, member 1, EC 3.4.21, EC 3.4.21.36, ELA1elastase-1, elastase 1, pancreatic, Elastase-1

Entrez Gene IDs

1990 (Human)

Gene Symbol

CELA1

Additional CELA1 Products

Product Documents for CELA1 Antibody (4H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CELA1 Antibody (4H5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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