CENPA Antibody (5A7-2E11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12892
![Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892]](https://resources.bio-techne.com/images/products/CENPA-Antibody-5A7-2E11-Western-Blot-NBP2-12892-img0002.jpg "Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5A7-2E11
Concentration
1 mg/ml
Product Summary for CENPA Antibody (5A7-2E11)
Immunogen
Synthetic peptide corresponding to a portion of human CENP-A
Localization
Nucleus , Chromosome , Centromere , Kinetochore
Specificity
Detects approx 18kDa. Recognizes Human CENP-A.
Marker
Centromeres Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CENPA Antibody (5A7-2E11)
Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892]
Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.![Immunocytochemistry/ Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892]](https://resources.bio-techne.com/images/products/CENPA-Antibody-5A7-2E11-Immunocytochemistry-Immunofluorescence-NBP2-12892-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892]")
Immunocytochemistry/ Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892]
Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.Applications for CENPA Antibody (5A7-2E11)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Application Notes
1 ug/ml of CENP-A Antibody was sufficient for detection of CENPA in 20 ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CENPA
Alternate Names
Centromere autoantigen A, centromere protein A (17kD), centromere protein ACENP-Acentromere protein A, 17kDa, histone H3-like centromeric protein A
Gene Symbol
CENPA
UniProt
Additional CENPA Products
Product Documents for CENPA Antibody (5A7-2E11)
Product Specific Notices for CENPA Antibody (5A7-2E11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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