CENPE Antibody (PSH03-54)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32180
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-54
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human CENPE aa 1-400 / 2,701. (Uniprot: Q02224)
Localization
Chromosome, centromere, kinetochore, Cytoplasm, cytoskeleton, spindle.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
316 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CENPE Antibody (PSH03-54)
Western Blot: CENPE Antibody (PSH03-54) [NBP3-32180]
Western Blot: CENPE Antibody (PSH03-54) [NBP3-32180] - Western blot analysis of CENPE on different lysates with Rabbit anti-CENPE antibody (NBP3-32180) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HEK-293 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 316 kDa
Observed band size: 316 kDa
Exposure time: 43 seconds;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for CENPE Antibody (PSH03-54)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CENPE
Long Name
Centromere-associated protein E
Alternate Names
CENP-E, Kinesin-7
Gene Symbol
CENPE
Additional CENPE Products
Product Documents for CENPE Antibody (PSH03-54)
Product Specific Notices for CENPE Antibody (PSH03-54)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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