CENPH Antibody (OTI2G4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72163

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CENPH Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CENPH(NP_075060) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CENPH Antibody (OTI2G4) - Azide and BSA Free

Western Blot: CENPH Antibody (OTI2G4)Azide and BSA Free [NBP2-72163]

Western Blot: CENPH Antibody (OTI2G4)Azide and BSA Free [NBP2-72163]

Western Blot: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH.
Immunohistochemistry: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163]

Immunohistochemistry: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163]

Immunohistochemistry: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163] - Staining of paraffin-embedded Human lung tissue using anti-CENPH mouse monoclonal antibody.
Flow Cytometry: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163]

Flow Cytometry: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163]

Flow Cytometry: CENPH Antibody (OTI2G4) - Azide and BSA Free [NBP2-72163] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CENPH antibody, and then analyzed by flow cytometry.
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Applications for CENPH Antibody (OTI2G4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CENPH

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Alternate Names

CENP-H, centromere protein H, ICEN35, Interphase centromere complex protein 35, kinetochore protein CENP-H, NNF1, PMF1

Gene Symbol

CENPH

Additional CENPH Products

Product Documents for CENPH Antibody (OTI2G4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CENPH Antibody (OTI2G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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