CENPJ Antibody (1A5)

Novus Biologicals, part of Bio-Techne | Catalog # H00055835-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CENPJ Antibodies »

Product Specifications

Immunogen

CENPJ (NP_060921, 979 a.a. ~ 1088 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQ

Specificity

CENPJ - centromere protein J (1A5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CENPJ Antibody (1A5)

Western Blot: CENPJ Antibody (1A5) [H00055835-M02]

Western Blot: CENPJ Antibody (1A5) [H00055835-M02]

Western Blot: CENPJ Antibody (1A5) [H00055835-M02] - Analysis of CENPJ expression in transfected 293T cell line by CENPJ monoclonal antibody (M02), clone 1A5.Lane 1: CENPJ transfected lysate(43.2 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: CENPJ Antibody (1A5) [H00055835-M02]

Immunoprecipitation: CENPJ Antibody (1A5) [H00055835-M02]

Immunoprecipitation: CENPJ Antibody (1A5) [H00055835-M02] - Analysis of CENPJ transfected lysate using anti-CENPJ monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPJ MaxPab rabbit polyclonal antibody.
Sandwich ELISA: CENPJ Antibody (1A5) [H00055835-M02] - Detection limit for recombinant GST tagged CENPJ is 0.1 ng/ml as a capture antibody.

Applications for CENPJ Antibody (1A5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPJ

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. [provided by RefSeq]

Alternate Names

BM032, CENP-J, centromere protein J, Centrosomal P4.1-associated protein, CPAPMCPH6, LAG-3-associated protein, LAPSCKL4, LIP1microcephaly, primary autosomal recessive 6, LYST-interacting protein 1, LYST-interacting protein LIP1, LYST-interacting protein LIP7, MGC131581, MGC131582, MGC142222, MGC142224, Sas-4, SASS4

Entrez Gene IDs

55835 (Human)

Gene Symbol

CENPJ

UniProt

NP_060921 (Human)

Additional CENPJ Products

Product Documents for CENPJ Antibody (1A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CENPJ Antibody (1A5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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