CENPJ Antibody (5D5)

Novus Biologicals, part of Bio-Techne | Catalog # H00055835-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5D5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CENPJ Antibodies »

Product Specifications

Immunogen

CENPJ (NP_060921, 979 a.a. ~ 1088 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQ

Reactivity Notes

Human. Other species not tested.

Specificity

CENPJ - centromere protein J

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CENPJ Antibody (5D5)

Immunohistochemistry-Paraffin: CENPJ Antibody (5D5) [H00055835-M01]

Immunohistochemistry-Paraffin: CENPJ Antibody (5D5) [H00055835-M01]

Immunohistochemistry-Paraffin: CENPJ Antibody (5D5) [H00055835-M01] - Analysis of monoclonal antibody to CENPJ on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 1.5 ug/ml.
Sandwich ELISA: CENPJ Antibody (5D5) [H00055835-M01] - Detection limit for recombinant GST tagged CENPJ is approximately 0.03ng/ml as a capture antibody.

Applications for CENPJ Antibody (5D5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPJ

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

Alternate Names

BM032, CENP-J, centromere protein J, Centrosomal P4.1-associated protein, CPAPMCPH6, LAG-3-associated protein, LAPSCKL4, LIP1microcephaly, primary autosomal recessive 6, LYST-interacting protein 1, LYST-interacting protein LIP1, LYST-interacting protein LIP7, MGC131581, MGC131582, MGC142222, MGC142224, Sas-4, SASS4

Entrez Gene IDs

55835 (Human)

Gene Symbol

CENPJ

OMIM

608393 (Human)

UniProt

NP_060921 (Human)

Additional CENPJ Products

Product Documents for CENPJ Antibody (5D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CENPJ Antibody (5D5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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