CENPK Antibody (1G2-1B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00064105-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G2-1B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CENPK Antibodies »

Product Specifications

Immunogen

CENPK (AAH05400.1, 1 a.a. ~ 269 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNGIALRHPEDPTRIRLEAFHQ

Reactivity Notes

Human. Other species not tested.

Specificity

FKSG14 - leucine zipper protein FKSG14

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CENPK Antibody (1G2-1B10)

Western Blot: CENPK Antibody (1G2-1B10) [H00064105-M01]

Western Blot: CENPK Antibody (1G2-1B10) [H00064105-M01]

Western Blot: CENPK Antibody (1G2-1B10) [H00064105-M01] - CENPK monoclonal antibody (M01), clone 1G2-1B10 Analysis of CENPK expression in HepG2.
Immunocytochemistry/ Immunofluorescence: CENPK Antibody (1G2-1B10) [H00064105-M01]

Immunocytochemistry/ Immunofluorescence: CENPK Antibody (1G2-1B10) [H00064105-M01]

Immunocytochemistry/Immunofluorescence: CENPK Antibody (1G2-1B10) [H00064105-M01] - Analysis of monoclonal antibody to CENPK on HepG2 cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CENPK Antibody (1G2-1B10) [H00064105-M01] - Detection limit for recombinant GST tagged FKSG14 is approximately 0.03ng/ml as a capture antibody.

Applications for CENPK Antibody (1G2-1B10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPK

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM]

Alternate Names

AF5alpha, CEMPK, CENP-KInterphase centromere complex protein 37, centromere protein K, FKSG14, ICEN37, leucine zipper protein FKSG14, P33, Protein AF-5alpha, SOLT, SoxLZ/Sox6-binding protein Solt

Entrez Gene IDs

64105 (Human)

Gene Symbol

CENPK

UniProt

AAH05400 (Human)

Additional CENPK Products

Product Documents for CENPK Antibody (1G2-1B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CENPK Antibody (1G2-1B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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