Centaurin beta 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1321

Novus Biologicals, part of Bio-Techne
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NB100-1321
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

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Product Specifications

Immunogen

Peptide with sequence C-EKLNRFQQDSQKF corresponding to C-Terminus according to NP_036419.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Centaurin beta 2 Antibody

Western Blot: Centaurin beta 2 Antibody [NB100-1321]

Western Blot: Centaurin beta 2 Antibody [NB100-1321]

Western Blot: Centaurin beta 2 Antibody [NB100-1321] - Staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Centaurin beta 2 Antibody [NB100-1321]

Immunohistochemistry-Paraffin: Centaurin beta 2 Antibody [NB100-1321]

Immunohistochemistry-Paraffin: Centaurin beta 2 Antibody [NB100-1321] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Centaurin beta 2 Antibody [NB100-1321]

Western Blot: Centaurin beta 2 Antibody [NB100-1321]

Western Blot: Centaurin beta 2 Antibody [NB100-1321] - (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Applications for Centaurin beta 2 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in human T-cell line MOLT4 lysates (calculated MW of 88.1 kDa band according to NP_036419). IHC-P: Human Lung shows strong staining of Type II pneumocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Centaurin beta 2

Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.

Alternate Names

Arf GAP with coiled coil, ANK repeat and PH domains 2, ArfGAP with coiled-coil, ankyrin repeat and PH domains 2, centaurin, beta 2, Centaurin-beta-2, CENTB2centaurin-beta-2, CNT-B2, KIAA0041arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Entrez Gene IDs

23527 (Human); 78618 (Mouse)

Gene Symbol

ACAP2

UniProt

NP_036419. (Human)

Additional Centaurin beta 2 Products

Product Documents for Centaurin beta 2 Antibody

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Certificate of Analysis

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Product Specific Notices for Centaurin beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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