CEP164 Antibody

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445]

Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - CEP295 overexpression induces extra-long microtubule-based filaments with low efficiency. Schematics on right of D indicate centrioles (black rectangles) and positions of CEP164 (red squares). MC, mother centriole; DC, daughter centriole. Image collected and cropped by CiteAb from the following publication (https://jcs.biologists.org/lookup/doi/10.1242/jcs.186338), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445]

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445]

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human bronchus shows moderate to strong positivity in cilia in respiratory epithelial cells.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445]

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human nasopharynx shows strong positivity in cilia in respiratory epithelial cells.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445]

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human endometrium shows strong positivity in cilia in glandular cells.

Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] -

Fetuin-A inhibits ciliogenesis. (A–D) Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. (E) Quantification results of the frequency of ciliated HTR8 cells. ** p < 0.01, results are the mean ± SD from three independent experiments, more than 100 cells were counted in each individual group. DNA was stained with DAPI. Scale bar 10 µM.

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] -

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. (A–D) Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. (E) Quantification results of the frequency of ciliated HTR8 cells. ** p < 0.01, results are the mean ± SD from three independent experiments, more than 100 cells were counted in each individual group. DNA was stained with DAPI. Scale bar 10 µM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31640125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] -

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Super-resolution (3D-SIM) microscopic analysis of centriolar distal-end proteins. (a) RPE1 cells were synchronized at G2 phase & immunostained with the indicated antibodies. Lower panels show fluorescence profile plots. Representative images of more than ten cells are presented in (a). ND: nascent daughter centriole, M: mother centriole. (b) The diameters of C2CD3, Talpid3, CEP120, & CEP164 protein spots (n = 30/each). Cumulative data from three independent experiments are shown. ***P < 0.001. (c,d) 3D-SIM analysis of the spatial localizations of C2CD3 (c), Talpid3 (d) & CEP120 (c,d) at the distal ends of centrioles. RPE1 cells were synchronized at G2 phase & immunostained with the indicated antibodies. Scale bar, 100 nm in (a,c,d). (e) Schematic illustration of the spatial localizations of C2CD3, Talpid3, CEP120, & CP110 at the distal ends of centrioles. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30988386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] -

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Depletion of RTTN produces shorter centrioles. a, b U2OS cells were treated with siControl or siRTTN as shown in a, & analyzed by confocal fluorescence microscopy using the indicated antibodies. cHistogram illustrating the distance between the CEP162-positive dots associated with a given pair of orthogonally oriented centrioles. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). d, f PLK4-myc doxycycline (Dox) inducible cells were treated with siControl or siRTTN as shown in a, & then analyzed by confocal fluorescence microscopy using the indicated antibodies d, or by electron microscopy f. e, gHistogram illustrating the length of procentrioles in PLK4-myc-inducible cells, as analyzed by confocal microscopy e or electron microscopy g. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). The procentriole length in b/c was measured as the distance between two CEP162 dots as described by Azimzadeh et al.17 The procentriole length in d/e was measured as the distance between the fluorescent peak intensity of SAS-6 (red) & CEP162 (green) in siControl & siRTTN-treated cells. Scale bar, 5 μm in b & d; Scale bar, 200 nm in f Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-017-00305-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] -

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - CPAP-E1235V mutation impaired the recruitment of centriole elongation proteins onto the centrioles. Cells were synchronized in the early S phase by treatment with aphidicolin (2 μg/ml) for 24 h. They were then fixed immediately with methanol (S phase centrioles) or released into fresh culture medium without aphidicolin for another 16 h (G2 phase centrioles). CPAP-WT or mutant cells in the S phase or G2 phase were immunostained with antibodies against the following centriolar proteins. (A) STIL, n = 42 for CPAP-WT; n = 25 for CPAP-E1235V#1; n = 34 for CPAP-E1235V#2. (B) CEP120, n = 50 for CPAP-WT; n = 42 for CPAP-E1235V#1; n = 48 for CPAP-E1235V#2. (C) CENTROBIN, n = 40 for all groups. (D) CEP295, n = 40 for all groups. (E) POC5, n = 42 for CPAP-WT; n = 40 for CPAP-E1235V#1; n = 40 for CPAP-E1235V#2. (F) POC1B, n = 40 for all groups. (G) CEP164, n = 88 for CPAP-WT; n = 74 for CPAP-E1235V#1; n = 38 for CPAP-E1235V#2. All data are presented as mean ±SEM from a pool of n cells from three independent experiments. n.s: not significant; **p < 0.01; ***p < 0.001. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35309908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] -

Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Depletion of RTTN produces shorter centrioles. a, b U2OS cells were treated with siControl or siRTTN as shown in a, & analyzed by confocal fluorescence microscopy using the indicated antibodies. cHistogram illustrating the distance between the CEP162-positive dots associated with a given pair of orthogonally oriented centrioles. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). d, f PLK4-myc doxycycline (Dox) inducible cells were treated with siControl or siRTTN as shown in a, & then analyzed by confocal fluorescence microscopy using the indicated antibodies d, or by electron microscopy f. e, gHistogram illustrating the length of procentrioles in PLK4-myc-inducible cells, as analyzed by confocal microscopy e or electron microscopy g. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). The procentriole length in b/c was measured as the distance between two CEP162 dots as described by Azimzadeh et al.17 The procentriole length in d/e was measured as the distance between the fluorescent peak intensity of SAS-6 (red) & CEP162 (green) in siControl & siRTTN-treated cells. Scale bar, 5 μm in b & d; Scale bar, 200 nm in f Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-017-00305-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.