CEP170 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55317

Novus Biologicals, part of Bio-Techne
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NB100-55317
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Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation Sequencing, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CEP170 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1534 and the C-teriminus (residue 1584) of human centrosomal protein 170kDa using the numbering given in entry NP_055627.2 (GeneID 9859).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEP170 Antibody

Western Blot: CEP170 Antibody [NB100-55317]

Western Blot: CEP170 Antibody [NB100-55317]

Western Blot: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (5, 15, and 50 ug) stained with CEP170 Antibody diluted at 0.04 ug/ml.
Immunocytochemistry/ Immunofluorescence: CEP170 Antibody [NB100-55317]

Immunocytochemistry/ Immunofluorescence: CEP170 Antibody [NB100-55317]

Immunocytochemistry/Immunofluorescence: CEP170 Antibody [NB100-55317] - NBF-fixed asynchronous HeLa cells, stained wtih CEP170 Antibody diluted at 1:200. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus(TM) 555.
Immunoprecipitation: CEP170 Antibody [NB100-55317]

Immunoprecipitation: CEP170 Antibody [NB100-55317]

Immunoprecipitation: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (1 mg) immunoprecipitated by rabbit anti-CEPT170 antibodies NB100-55317 at 3 ug/mg lysate.CEP170 was also immunoprecipitated by rabbit anti-CEP170 antibodies NB100-55317 and BL4463, which recognize upstream epitopes.

Applications for CEP170 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
For ICC/IF: Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CEP170

The product of this gene is a component of the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. The protein interacts with and is phosphorylated by polo-like kinase 1, and functions in maintaining microtubule organization and cell morphology. The human genome contains a putative transcribed pseudogene. Several alternatively spliced transcript variants of this gene have been found, but the full-length nature of some of these variants has not been determined.

Alternate Names

centrosomal protein 170kDa, Cep170, KARP 1 binding protein, KARP-1-binding protein, KARP1-binding protein, KIAA0470FAM68AKABcentrosomal protein of 170 kDa, XRCC5 binding protein

Entrez Gene IDs

9859 (Human)

Gene Symbol

CEP170

UniProt

Q5SW79 (Human)

Additional CEP170 Products

Product Documents for CEP170 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CEP170 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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