Ceramide Kinase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2911

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.82 mg/ml

View all Ceramide Kinase Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of human Ceramide Kinase (between residues 50-150). [Swiss-Prot# Q8TCT0]

Localization

Cytoplasmic and membraneous, cytoplasmic vesicles

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ceramide Kinase Antibody

Western Blot: Ceramide Kinase Antibody [NB100-2911]

Western Blot: Ceramide Kinase Antibody [NB100-2911]

Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.
Immunocytochemistry/ Immunofluorescence: Ceramide Kinase Antibody [NB100-2911]

Immunocytochemistry/ Immunofluorescence: Ceramide Kinase Antibody [NB100-2911]

Immunocytochemistry/Immunofluorescence: Ceramide Kinase Antibody [NB100-2911] - Ceramide Kinase antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Applications for Ceramide Kinase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Western Blot

2 ug/ml
Application Notes
This Ceramide Kinase antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is seen at ~65 kDa representing the Ceramide Kinase protein, and in Immunocytochemistry/Immunofluorescence vesicular cytoplasmic staining was observed in A431 cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ceramide Kinase

Ceramide kinase (CERK) belongs to diacylglycerol kinase family of lipid kinases and it catalyzes specifically the phosphorylation of ceramide to generate ceramide 1-phosphate (C1P). CERK acts primarily on natural as well as analog ceramides such as C6, C8, C16 ceramides, and C8-dihydroceramide, to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids including sphingosines. Localized in the cytoplasm and membrane as peripheral membrane protein, CERK is the only enzyme known to produce C1P, a signaling molecule which shows positive effect on cell proliferation or survival and also regulates cytosolic phospholipase A2 (cPLA2) activity . CERK/C1P signaling is implicated in mast cell activation, eicosanoid signaling, apoptosis, cellular immune response/inflammation, emotional behavior, angiogenesis, phagolysosome formation etc.

Long Name

Ceramide kinase

Alternate Names

CERK, EC 2.7.1.138, hCERK, KIAA1646, Lipid kinase 4, LK4

Gene Symbol

CERK

Additional Ceramide Kinase Products

Product Documents for Ceramide Kinase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ceramide Kinase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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