Ceruloplasmin Antibody (JE59-01)

Western Blot: Ceruloplasmin Antibody (JE59-01) [NBP3-32182]

Western Blot: Ceruloplasmin Antibody (JE59-01) [NBP3-32182] - Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (NBP3-32182) at 1/1,000 dilution.

Lane 1: Rat liver tissue lysate
Lane 2: Rat testis tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 122 kDa
Observed band size: 140 kDa

Exposure time: 3 minutes 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32182) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.