Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM13-4

Format

BSA Free

Concentration

1 mg/ml

View all CFTR Antibodies »

Product Specifications

Immunogen

Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR).

Reactivity Notes

Does not work on mouse or dogfish shark.

Specificity

Human cystic fibrosis transmembrane conductance regulator (CFTR). Recognizes an epitope at the N-terminal between residues 24 and 35.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Relevant Entrez Gene Numbers: NM_000492.3

Applications for CFTR Antibody (MM13-4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

10 ug/ml
Application Notes
Western Blot:It recognizes CFTR (150-170kDa) and two additional proteins at 52kDa and 38kDa Note: Does not work on paraffin embedded tissue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

20mM Phosphate (pH 7.6) and 0.25M NaCl

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CFTR

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

Long Name

Cystic Fibrosis Transmembrane Conductance Regulator

Alternate Names

ABC35, ABCC7CF, ATP-binding cassette sub-family C member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7), cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7), dJ760C5.1, EC 3.6.3, MRP7EC 3.6.3.49, TNR-CFTR

Gene Symbol

CFTR

UniProt

P13569

Additional CFTR Products

Product Documents for CFTR Antibody (MM13-4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CFTR Antibody (MM13-4) - BSA Free

Relevant Entrez Gene Numbers: NM_000492.3

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.