Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CGI-09 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 447 and 497 of human tRNA Methyltransferase 6 using the numbering given in entry NP_057023.2 (GeneID 51605).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CGI-09 Antibody

Western Blot: CGI-09 Antibody [NBP1-50040]

Western Blot: CGI-09 Antibody [NBP1-50040]

Western Blot: CGI-09 Antibody [NBP1-50040] - Detection of Human TRM6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-TRM6 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. TRM6 was also immunoprecipitated by rabbit anti-TRM6 antibody which recognizes an upstream epitope. For blotting immunoprecipitated TRM6, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for CGI-09 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CGI-09

The protein encoded by this gene is similar to an uncharacterized protein from C. elegans. Although the function of the encoded protein is unknown, it shares weak sequence similarity to a region of S. cerevisiae translation initiation factor subunit Gcd10p.

Alternate Names

CGI-09, GCD10, Gcd10p, KIAA1153, MGC5029, TRM6, tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6, tRNA methyltransferase 6 homolog (S. cerevisiae), tRNA(m1A58)-methyltransferase subunit TRM6, tRNA(m1A58)MTase subunit TRM6

Gene Symbol

TRMT6

Additional CGI-09 Products

Product Documents for CGI-09 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CGI-09 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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