cGK1/PRKG1 Antibody (5E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005592-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all cGK1/PRKG1 Antibodies »

Product Specifications

Immunogen

PRKG1 (NP_006249, 73 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGV

Specificity

PRKG1 - protein kinase, cGMP-dependent, type I

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for cGK1/PRKG1 Antibody (5E5)

Western Blot: cGK1/PRKG1 Antibody (5E5) [H00005592-M01]

Western Blot: cGK1/PRKG1 Antibody (5E5) [H00005592-M01]

Western Blot: cGK1/PRKG1 Antibody (5E5) [H00005592-M01] - Analysis of PRKG1 expression in transfected 293T cell line by PRKG1 monoclonal antibody (M01), clone 5E5.Lane 1: PRKG1 transfected lysate(35.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: cGK1/PRKG1 Antibody (5E5) [H00005592-M01] - Detection limit for recombinant GST tagged PRKG1 is approximately 0.03ng/ml as a capture antibody.

Applications for cGK1/PRKG1 Antibody (5E5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: cGK1/PRKG1

cGK1/PRKG1, also known as cGMP-dependent protein kinase 1, has two isoforms that are produced by alternative splicing. Isoform Alpha (CGK1-alpha) is 671 amino acids long and 76 kDa. Isoform Beta (CGK1-beta) is 686 amino acids long and approximately 78 kDa. PRKG1 is a Serine/threonine protein kinase that is expressed in placenta and lung. cGK1/PRKG1 is an important player in the Nitric Oxide/cGMP Signaling Pathway; the binding of GMP activates PRKG1, which in turn phosphorylates Serine residues and Threonine residues on various cell proteins, including proteins that regulate calcium levels, smooth muscle contraction, platelet adhesion, circadian rhythm and more. Current research on PRKG1 is being conducted in relation to several diseases and disorders including cystic fibrosis, lymphoma, non-small cell lung carcinoma, Wiskott-Aldrich syndrome, Waterhouse-Friderichsen syndrome and myoglobinuria. PRKG1 has also been shown to have interactions with SMAD4, BMPR2, GKAP1, Natriuretic Peptide Receptor A and TFII-I in pathways such as Gap Junction signaling, eNOS signaling and EDNRB signaling.

Long Name

cGMP-dependent protein kinase 1

Alternate Names

cGK 1, cGK1, cGKI, EC 2.7.11.12, PRKG1, PRKG1B, PRKGR1A, PRKGR1B

Entrez Gene IDs

5592 (Human)

Gene Symbol

PRKG1

OMIM

176894 (Human)

UniProt

NP_006249 (Human)

Additional cGK1/PRKG1 Products

Product Documents for cGK1/PRKG1 Antibody (5E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for cGK1/PRKG1 Antibody (5E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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